Endogenous cholecystokinin, the major factor responsible for dietary protein-induced pancreatic growth

Pancreas. 1992;7(5):522-9. doi: 10.1097/00006676-199209000-00003.

Abstract

This study was undertaken to clarify the role of endogenous cholecystokinin (CCK) in induction of pancreatic growth stimulated by a high protein diet. Rats with i.v. jugular cannulae in place and kept in Bollman cages were adapted to 5% casein diet for 9 days and switched to 70% casein for 2 days. MK-329, a CCK receptor antagonist, and SMS 201-995, a somatostatin agonist, were continuously infused at 0.5 mg/kg/h and 5 micrograms/kg/h, respectively, starting at the onset of feeding 70% casein. The 5 and 70% casein control groups were infused with saline. Feeding 70% casein significantly stimulated pancreatic hyperplasia and tissue hypertrophy. MK-329 and SMS 201-995 totally prevented 70% casein-induced increases in pancreatic weight and total RNA and DNA contents. The results indicate that endogenous CCK is the major factor responsible for pancreatic growth induced by a high protein diet.

Publication types

  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Amylases / metabolism
  • Animals
  • Benzodiazepinones / pharmacology
  • Body Weight
  • Caseins / administration & dosage
  • Caseins / pharmacology
  • Cholecystokinin / physiology*
  • Chymotrypsinogen / metabolism
  • DNA / metabolism
  • Devazepide
  • Dietary Proteins / pharmacology*
  • Hyperplasia
  • Octreotide / pharmacology
  • Organ Size
  • Pancreas / drug effects
  • Pancreas / pathology*
  • Proteins / metabolism
  • RNA / metabolism
  • Rats
  • Rats, Inbred Strains
  • Receptors, Cholecystokinin / antagonists & inhibitors

Substances

  • Benzodiazepinones
  • Caseins
  • Dietary Proteins
  • Proteins
  • Receptors, Cholecystokinin
  • RNA
  • DNA
  • Cholecystokinin
  • Chymotrypsinogen
  • Amylases
  • Devazepide
  • Octreotide