High spontaneous granulocyte/macrophage-colony formation in patients with myelofibrosis

Verena Hauer; Eva Jäger; Thamer Sliwa; Ralph Simanek; Heinz Gisslinger; Ulrich Jäger; Klaus Geissler

doi:10.1016/j.leukres.2013.10.003

High spontaneous granulocyte/macrophage-colony formation in patients with myelofibrosis

Leuk Res. 2014 Jan;38(1):116-20. doi: 10.1016/j.leukres.2013.10.003. Epub 2013 Oct 14.

Authors

Verena Hauer¹, Eva Jäger², Thamer Sliwa¹, Ralph Simanek¹, Heinz Gisslinger³, Ulrich Jäger³, Klaus Geissler⁴

Affiliations

¹ 5th Department of Internal Medicine - Oncology/Hematology, Hospital Hietzing, Vienna, Austria.
² Department of Laboratory Medicine, Medical University of Vienna, Vienna, Austria.
³ Department of Internal Medicine I, Division of Hematology and Hemostaseology, Medical University of Vienna, Vienna, Austria.
⁴ 5th Department of Internal Medicine - Oncology/Hematology, Hospital Hietzing, Vienna, Austria; Ludwig Boltzmann Institute for Clinical Oncology, Vienna, Austria. Electronic address: klaus.geissler@wienkav.at.

PMID: 24262286
DOI: 10.1016/j.leukres.2013.10.003

Abstract

Unstimulated methylcellulose cultures in 25 myelofibrosis (MF) patients were performed to better understand the role of cytokines in the proliferation of MF cells. Compared to controls MF patients show a variable but highly increased spontaneous CFU-GM formation (66 vs 4.8/10(5) PBMNC). There was a marked reduction of autonomous CFU-GM growth by the cytokine-synthesis-inhibiting molecule IL-10 as well as by antibodies against GM-CSF whereas antibodies against IL-3, G-CSF, M-CSF and IL-1β showed heterogeneous effects. Spontaneous CFU-GM growth >100/10(5) PBMNC predicted shorter survival. Constitutive release of GM-CSF seems to contribute to proliferation of MF cells in vitro and possibly in vivo.

Keywords: CFU-GM; Cultures; Methylcellulose; Myelofibrosis; Spontaneous.

MeSH terms

Antibodies, Neutralizing / immunology
Antibodies, Neutralizing / pharmacology
Cell Culture Techniques
Cell Proliferation / drug effects
Cells, Cultured
Colony-Forming Units Assay / methods*
Granulocyte Colony-Stimulating Factor / immunology
Granulocyte Colony-Stimulating Factor / metabolism
Granulocyte Colony-Stimulating Factor / pharmacology
Granulocyte-Macrophage Colony-Stimulating Factor / immunology
Granulocyte-Macrophage Colony-Stimulating Factor / metabolism
Granulocyte-Macrophage Colony-Stimulating Factor / pharmacology
Granulocyte-Macrophage Progenitor Cells / drug effects
Granulocyte-Macrophage Progenitor Cells / metabolism
Granulocyte-Macrophage Progenitor Cells / pathology*
Interleukin-10 / metabolism
Interleukin-10 / pharmacology
Interleukin-1beta / immunology
Interleukin-1beta / metabolism
Interleukin-1beta / pharmacology
Interleukin-3 / immunology
Interleukin-3 / metabolism
Interleukin-3 / pharmacology
Kaplan-Meier Estimate
Leukocytes, Mononuclear / drug effects
Leukocytes, Mononuclear / metabolism
Leukocytes, Mononuclear / pathology*
Macrophage Colony-Stimulating Factor / immunology
Macrophage Colony-Stimulating Factor / metabolism
Macrophage Colony-Stimulating Factor / pharmacology
Primary Myelofibrosis / blood
Primary Myelofibrosis / metabolism
Primary Myelofibrosis / pathology*

Substances

Antibodies, Neutralizing
Interleukin-1beta
Interleukin-3
Interleukin-10
Granulocyte Colony-Stimulating Factor
Macrophage Colony-Stimulating Factor
Granulocyte-Macrophage Colony-Stimulating Factor