Abstract
In vitro labeling of arterial wall samples from domestic swine with tritiated thymidine or proline was used to establish the effect of unsaturated (sunflower oil) or saturated (beef tallow) fatty acids in the presence of enteral cholesterol on DNA and collagen synthesis. Histoautoradiography of samples incubated in vitro gives labeling patterns and indices comparable to in vivo labeling. Preliminary results show that these nutrient fats do not differ in their effect on the DNA and collagen synthesis of vascular wall cells both in the presence and absence of intimal changes.
Publication types
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Comparative Study
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Research Support, Non-U.S. Gov't
MeSH terms
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Animals
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Arteries / analysis
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Arteries / drug effects
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Arteries / metabolism*
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Arteriosclerosis / metabolism*
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Arteriosclerosis / pathology
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Autoradiography
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Cholesterol, Dietary / administration & dosage
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Cholesterol, Dietary / pharmacology*
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Collagen / biosynthesis*
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DNA / biosynthesis*
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Diet, Atherogenic
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Fats / administration & dosage
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Fats / pharmacology
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Fatty Acids / administration & dosage
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Fatty Acids / pharmacology
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Fatty Acids, Unsaturated / administration & dosage
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Fatty Acids, Unsaturated / pharmacology
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Female
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Plant Oils / administration & dosage
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Plant Oils / pharmacology
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Proline / analysis
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Sunflower Oil
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Swine
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Thymidine / analysis
Substances
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Cholesterol, Dietary
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Fats
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Fatty Acids
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Fatty Acids, Unsaturated
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Plant Oils
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Sunflower Oil
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Collagen
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DNA
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tallow
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Proline
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Thymidine