Protocol for Patch-Seq of Small Interneurons

Marcela Lipovsek; Lorcan Browne; Matthew S Grubb

doi:10.1016/j.xpro.2020.100146

Protocol for Patch-Seq of Small Interneurons

STAR Protoc. 2020 Oct 22;1(3):100146. doi: 10.1016/j.xpro.2020.100146. eCollection 2020 Dec 18.

Authors

Marcela Lipovsek¹, Lorcan Browne¹, Matthew S Grubb¹

Affiliation

¹ Centre for Developmental Neurobiology, Institute of Psychiatry, Psychology and Neuroscience (IoPPN), King's College London, London, UK.

Abstract

Obtaining electrophysiological recordings and gene expression information from the same neuron (Patch-seq) brings forward a unique opportunity to study the transcriptional correlates of functional properties and vice versa. Here, we provide a detailed Patch-seq protocol tailored to the specialized demands of studying small interneurons. Focusing on the technically demanding process of transitioning between patch recordings and cell extraction, our protocol describes and troubleshoots steps for successfully collecting small interneurons, allowing for multi-modal Patch-seq interrogation of this crucial cell type.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Electrophysiological Phenomena / genetics
Electrophysiological Phenomena / physiology
Gene Expression / genetics
Gene Expression Profiling / methods*
Interneurons / cytology*
Interneurons / metabolism
Interneurons / physiology
Neurons / metabolism
Patch-Clamp Techniques / methods*
Sequence Analysis, RNA / methods
Single-Cell Analysis / methods
Transcriptome / genetics