Studying the microglia response to oxidized phosphatidylcholine in primary mouse neuron culture and mouse spinal cord

Yifei Dong; Brian M Lozinski; Claudia Silva; V Wee Yong

doi:10.1016/j.xpro.2021.100853

Studying the microglia response to oxidized phosphatidylcholine in primary mouse neuron culture and mouse spinal cord

STAR Protoc. 2021 Sep 27;2(4):100853. doi: 10.1016/j.xpro.2021.100853. eCollection 2021 Dec 17.

Authors

Yifei Dong¹, Brian M Lozinski¹, Claudia Silva¹, V Wee Yong¹

Affiliation

¹ Hotchkiss Brain Institute and the Department of Clinical Neuroscience, University of Calgary, Calgary, AB T2N 4N1, Canada.

Abstract

Oxidized phosphatidylcholine (OxPC) found in multiple sclerosis brain lesions mediates neurodegeneration. Microglia are prominent responders to the OxPC insult, and thus, studying their protective or noxious functions is important to help halt neurodegeneration. Here, we present protocols including cell isolation and culture, animal surgeries, as well as tissue processing and isolation to study the microglia response to OxPC-mediated neurodegeneration in vitro and in vivo. For complete details on the use and execution of this protocol, please refer to Dong et al. (2021).

Keywords: Cell culture; Cell isolation; Cell-based Assays; Immunology; Microscopy; Model Organisms; Neuroscience.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Separation / methods
Lecithins
Mice
Microglia*
Neurons*
Spinal Cord

Substances

Lecithins

Grants and funding

CIHR/Canada