Abstract
p73 is a recently discovered homologue of the tumour suppressor p53 and contains all three functional domains of p53. The alpha-splice variant of p73 (p73alpha) contains an additional structural domain near its C--terminus that has sequence homology with the sterile alpha-motif (SAM) domain. This domain is considered to be responsible for mediating protein-protein interactions. Pyramidal crystals of human p73alpha SAM domain were obtained by the hanging-drop vapour-diffusion method with ammonium dihydrogen orthophosphate as the precipitant. The crystals diffract to 2.54 A resolution and belong to the tetragonal space group P4(1)2(1)2, with unit-cell parameters a = b = 32.02, c = 133.84 A. The structure was solved by molecular replacement using the NMR structure of the same protein as the search model.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Amino Acid Motifs
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Crystallization
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Crystallography, X-Ray
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DNA-Binding Proteins / biosynthesis
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DNA-Binding Proteins / chemistry*
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DNA-Binding Proteins / genetics
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DNA-Binding Proteins / isolation & purification
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Genes, Tumor Suppressor
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Genetic Vectors / chemical synthesis
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Humans
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Nuclear Magnetic Resonance, Biomolecular
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Nuclear Proteins / biosynthesis
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Nuclear Proteins / chemistry*
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Nuclear Proteins / genetics
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Nuclear Proteins / isolation & purification
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Peptide Fragments / chemistry
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Peptide Fragments / genetics
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Peptide Fragments / isolation & purification
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Protein Structure, Tertiary
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Tumor Protein p73
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Tumor Suppressor Proteins
Substances
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DNA-Binding Proteins
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Nuclear Proteins
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Peptide Fragments
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TP73 protein, human
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Tumor Protein p73
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Tumor Suppressor Proteins