Abstract
A 3' terminal RNA uridylyltransferase was purified from mitochondria of Leishmania tarentolae and the gene cloned and expressed from this species and from Trypanosoma brucei. The enzyme is specific for 3' U-addition in the presence of Mg(2+). TUTase is present in vivo in at least two stable configurations: one contains a approximately 500 kDa TUTase oligomer and the other a approximately 700 kDa TUTase complex. Anti-TUTase antiserum specifically coprecipitates a small portion of the p45 and p50 RNA ligases and approximately 40% of the guide RNAs. Inhibition of TUTase expression in procyclic T. brucei by RNAi downregulates RNA editing and appears to affect parasite viability.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Animals
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Chromatography, Ion Exchange
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Leishmania / cytology
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Leishmania / enzymology*
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Leishmania / genetics
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Magnesium / metabolism
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Mitochondria / chemistry
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Mitochondria / enzymology*
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Molecular Sequence Data
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Molecular Weight
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RNA / genetics
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RNA / metabolism
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RNA Editing*
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RNA Nucleotidyltransferases / genetics
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RNA Nucleotidyltransferases / isolation & purification*
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RNA Nucleotidyltransferases / metabolism*
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Trypanosoma brucei brucei / cytology
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Trypanosoma brucei brucei / enzymology*
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Trypanosoma brucei brucei / genetics
Substances
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Recombinant Proteins
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RNA
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RNA Nucleotidyltransferases
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UTP-RNA uridylyltransferase
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Magnesium
Associated data
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GENBANK/AY029069
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GENBANK/AY029070