Abstract
Peptides derived from the N- (N-HR) and C- (C-HR) terminal heptad repeat regions adjacent to the fusion peptide and transmembrane domains, respectively, of human immunodeficiency virus (HIV)-1 gp41 inhibit HIV-1 viral envelope glycoproteins (Env)-mediated cell fusion specifically. The mechanism of HIV-1 Env-mediated cell fusion and its inhibition by agents that target the N- and C-HR regions was investigated. Priming experiments with Env-expressing cells indicate that the N-HR region but not the C-HR region is exposed by treatment with sCD4 at 31 degrees C, whereas both the N- and C-HR regions are exposed at 37 degrees C.
Publication types
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Research Support, U.S. Gov't, P.H.S.
MeSH terms
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Amino Acid Sequence
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Animals
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CD4 Antigens / immunology
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CD4 Antigens / metabolism
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CHO Cells
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Cell Line
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Cricetinae
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Cricetulus
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HIV Envelope Protein gp41 / chemistry
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HIV Envelope Protein gp41 / metabolism*
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HIV Fusion Inhibitors / pharmacology*
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HIV-1 / metabolism*
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HeLa Cells
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Humans
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Membrane Fusion / drug effects
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Mice
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Molecular Sequence Data
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NIH 3T3 Cells
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Peptide Fragments / pharmacology*
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Protein Structure, Secondary
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Receptors, CXCR4 / metabolism
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Temperature
Substances
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CD4 Antigens
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HIV Envelope Protein gp41
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HIV Fusion Inhibitors
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Peptide Fragments
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Receptors, CXCR4