2'-Amino-2'-deoxyguanosine is a cofactor for self-splicing in group I catalytic RNA

Biochem Biophys Res Commun. 1992 Mar 16;183(2):842-8. doi: 10.1016/0006-291x(92)90560-8.

Abstract

In investigations on self-splicing in the group I intron of the pre-mRNA from the nrdB gene of bacteriophage T4 it was found that 2'-amino-2'-deoxyguanosine can replace guanosine as cofactor. This is the first guanosine-analogue with a modification in the 2'-position and substantial activity in a group I self-splicing reaction. The results suggest that the 2'-amino and 2'-hydroxy groups of the cosubstrates have some properties in common, which are important for binding as well as for catalysis.

Publication types

  • Comparative Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Deoxyguanosine / analogs & derivatives*
  • Deoxyguanosine / metabolism
  • Guanosine / analogs & derivatives
  • Guanosine / metabolism*
  • Introns
  • RNA Precursors / metabolism*
  • RNA, Catalytic / metabolism*
  • Structure-Activity Relationship
  • Substrate Specificity
  • T-Phages / metabolism*

Substances

  • RNA Precursors
  • RNA, Catalytic
  • Guanosine
  • 2'-amino-2'-deoxyguanosine
  • Deoxyguanosine