Time dependent rate of diffusion-influenced ligand binding to receptors on cell surfaces

Biophys J. 1991 Sep;60(3):671-8. doi: 10.1016/S0006-3495(91)82096-3.

Abstract

The theory of the kinetics of binding of ligands to a sphere partially covered by receptors is extended to provide the full time dependence of the reactive flux. The ligands diffuse to the receptors; the receptors are either fully or partially absorbing. The total flux into the sphere with many receptors is expressed analytically in terms of the flux into a single isolated receptor on the sphere. At steady state, the Berg-Purcell formula is generalized to the case where the binding to a single receptor is only partially diffusion controlled. At short times, the receptors behave independently and the total flux is the sum of the fluxes to the isolated receptors.

MeSH terms

  • Cell Membrane / metabolism*
  • Kinetics
  • Ligands
  • Mathematics
  • Models, Biological*
  • Protein Binding
  • Receptors, Cell Surface / metabolism*

Substances

  • Ligands
  • Receptors, Cell Surface