Analysis of the beta-tubulin gene from Vittaforma corneae suggests benzimidazole resistance

Antimicrob Agents Chemother. 2008 Feb;52(2):790-3. doi: 10.1128/AAC.00928-07. Epub 2007 Dec 3.

Abstract

We amplified, cloned, and sequenced the beta-tubulin gene of Vittaforma corneae, a microsporidium causing human infections. The beta-tubulin gene sequence has a substitution at Glu(198) (with glutamine), which is one of six amino acids reported to be associated with benzimidazole sensitivity. Benzimidazoles were assayed for antimicrosporidial activity and showed poor parasite inhibition.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amino Acid Sequence
  • Antifungal Agents / pharmacology*
  • Benzimidazoles / pharmacology*
  • Cell Line
  • Cloning, Molecular
  • Drug Resistance, Fungal / genetics*
  • Fibroblasts / microbiology
  • Humans
  • Microbial Sensitivity Tests
  • Microsporidiosis / microbiology
  • Molecular Sequence Data
  • Sequence Analysis, DNA
  • Tubulin / chemistry
  • Tubulin / genetics*
  • Tubulin / metabolism
  • Vittaforma / drug effects*
  • Vittaforma / genetics

Substances

  • Antifungal Agents
  • Benzimidazoles
  • Tubulin
  • benzimidazole

Associated data

  • GENBANK/EU031749