Oxidative DNA damage in patients with cataract

Acta Ophthalmol. 2010 Dec;88(8):891-5. doi: 10.1111/j.1755-3768.2009.01583.x.

Abstract

Purpose: This study examines the levels of oxidative damage in patients with cataract.

Methods: Blood samples were collected from 60 patients with cataract and 60 age- and gender-matched healthy individuals to measure 8-hydroxy 2-deoxyguanosine (8-OHdG) and malondialdehyde (MDA) levels.

Results: A significant difference was observed in leukocyte 8-OHdG levels in patients with cataract in comparison with healthy persons (p < 0.001). Similarly, a significant difference was observed in plasma MDA levels in patients with cataract in comparison with healthy persons (p<0.001). In addition, a significant correlation was found between levels of 8-OHdG in leukocyte DNA and plasma MDA (r = 0.859, p < 0.001).

Conclusion: This study measured the oxidative DNA damage by measuring the 8-OHdG in the leukocyte DNA in patients with cataract. In addition, the level of MDA - a marker for lipid peroxidation - was measured to determine lipid peroxidation.

MeSH terms

  • 8-Hydroxy-2'-Deoxyguanosine
  • Aged
  • Aging / physiology
  • Cataract / blood
  • Cataract / genetics*
  • Chromatography, High Pressure Liquid
  • DNA Damage*
  • Deoxyguanosine / analogs & derivatives
  • Deoxyguanosine / blood
  • Female
  • Humans
  • Leukocytes / metabolism
  • Lipid Peroxidation
  • Male
  • Malondialdehyde / blood
  • Middle Aged
  • Oxidative Stress*
  • Reactive Oxygen Species / metabolism

Substances

  • Reactive Oxygen Species
  • Malondialdehyde
  • 8-Hydroxy-2'-Deoxyguanosine
  • Deoxyguanosine