Abstract
Ovariectomy (OVX)-induced estrogen withdrawal resulted in both bone loss and an increase in fat. We observed elevated osteoclast (OC) formation by bone marrow-derived macrophages treated with medium conditioned by fats from OVX mice, but not from sham-operated mice. Fats from OVX mice expressed and secreted higher levels of monocyte chemoattractant protein-1 (MCP-1) than those from sham-operated mice. Increased fat resulting from estrogen deficiency is thus responsible for bone loss due to enhanced OC formation, which is, at least partly, a consequence of elevated MCP-1 production.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adipose Tissue / metabolism*
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Adipose Tissue / pathology
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Animals
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Bone Density
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Bone Diseases, Metabolic / etiology*
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Bone Diseases, Metabolic / pathology
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Bone Diseases, Metabolic / physiopathology
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Bone Resorption / physiopathology*
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Cell Differentiation / physiology
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Cells, Cultured / cytology
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Cells, Cultured / drug effects
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Chemokine CCL2 / biosynthesis
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Chemokine CCL2 / genetics
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Chemokine CCL2 / physiology*
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Estrogens / deficiency*
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Female
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Femur / chemistry
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Femur / pathology
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Intra-Abdominal Fat / metabolism
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Intra-Abdominal Fat / pathology
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Macrophage Colony-Stimulating Factor / physiology
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Macrophages / cytology
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Macrophages / drug effects
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Mice
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Mice, Inbred C57BL
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Osteoclasts / pathology*
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Ovariectomy
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RANK Ligand / physiology
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Recombinant Proteins / pharmacology
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Subcutaneous Fat / metabolism
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Subcutaneous Fat / pathology
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Weight Gain
Substances
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Ccl2 protein, mouse
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Chemokine CCL2
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Estrogens
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RANK Ligand
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Recombinant Proteins
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Tnfsf11 protein, mouse
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Macrophage Colony-Stimulating Factor