A nuclear magnetic resonance study of the hydrogen-exchange behaviour of lysozyme in crystals and solution

J Mol Biol. 1991 Mar 20;218(2):413-26. doi: 10.1016/0022-2836(91)90722-i.

Abstract

Amide hydrogen/deuterium exchange behaviour has been studied for all of the peptide amides of hen lysozyme by means of two-dimensional n.m.r. spectroscopy. The amides have been grouped into four categories on the basis of their rates of exchange in solution at pH 4.2 and 7.5. The distribution of the amides into the different categories has been examined in the light of the crystallographic structural information, considering the type of secondary structure, the nature of hydrogen bonding and the distance from the protein surface. None of these features was found to determine uniquely the pattern of hydrogen exchange rates within the protein. The exchange behaviour of the individual amides could, however, in general be rationalized by a combination of these features. Hydrogen exchange was also monitored in both tetragonal and triclinic crystals of lysozyme, by allowing exchange to take place in the crystals prior to dissolution and recording of n.m.r. spectra under conditions where further exchange was minimized. This enabled direct comparison to be made of the exchange behaviour in the crystals and solution. A reduction in exchange rate was observed in the crystalline state relative to solution for a substantial number of amides and distinct differences between exchange in the different crystals could be observed. These differences between the solution and the different crystal states do not, however, correlate in a simple manner with proximity to intermolecular contacts in the crystals. However, the existence of these contacts, which are on the surface of the protein molecule, have a profound effect on the exchange of amides in the interior of the protein. The results indicate that the spectrum of fluctuations giving rise to hydrogen exchange may be significantly altered by the intermolecular interactions present within the crystalline state.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Amides / chemistry
  • Animals
  • Chickens
  • Deuterium / chemistry
  • Hydrogen / chemistry*
  • Hydrogen Bonding
  • Hydrogen-Ion Concentration
  • Magnetic Resonance Spectroscopy
  • Muramidase / chemistry*
  • Protein Conformation
  • Solutions
  • X-Ray Diffraction

Substances

  • Amides
  • Solutions
  • Hydrogen
  • Deuterium
  • Muramidase