Opposite effects of Trichostatin A on activation of mast cells by different stimulants

FEBS Lett. 2010 Jun 3;584(11):2315-20. doi: 10.1016/j.febslet.2010.03.047. Epub 2010 Apr 4.

Abstract

Mast cells (MCs) are activated upon stimulation via TLRs or FcepsilonRI, contributing to immune protection and/or leading to allergic diseases. In the present study, the effects of Trichostatin A (TSA) on the activation of MCs were analyzed with bone marrow-derived (BM) MCs. TSA increased the transcription and protein secretion of IL-6 in case of LPS-stimulation, in contrast to the suppressive effect on IgE-mediated activation of BMMCs. Chromatin immunoprecipitation assay showed IgE-mediated signaling-specific suppression of transcription factors recruitment to the IL-6 promoter. TSA-treatment inhibited nuclear translocation of NF-kappaB following IgE-mediated, but not LPS-induced activation in MCs.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Hydroxamic Acids / pharmacology*
  • Interleukin-6 / genetics*
  • Interleukin-6 / metabolism*
  • Lipopolysaccharides / pharmacology
  • Mast Cells / drug effects
  • Mast Cells / metabolism*
  • NF-kappa B / genetics
  • NF-kappa B / metabolism*
  • Signal Transduction / drug effects
  • Transcription Factors / genetics
  • Transcription Factors / metabolism

Substances

  • Hydroxamic Acids
  • Interleukin-6
  • Lipopolysaccharides
  • NF-kappa B
  • Transcription Factors
  • trichostatin A