Abstract
Trypanosoma brucei imports all mitochondrial transfer RNAs (tRNAs) from the cytosol. By using cell lines that allow independent tetracycline-inducible RNA interference and isopropyl-β-D-thiogalactopyranoside-inducible expression of a tagged tRNA, we show that ablation of Tim17 and mitochondrial heat-shock protein 70, components of the inner-membrane protein translocation machinery, strongly inhibits import of newly synthesized tRNAs. These findings, together with previous results in yeast and plants, suggest that the requirement for mitochondrial protein-import factors might be a conserved feature of mitochondrial tRNA import in all systems.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Cell Line
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Cytosol / metabolism
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HSP70 Heat-Shock Proteins / metabolism
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Isopropyl Thiogalactoside / pharmacology
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Mitochondria / genetics
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Mitochondria / metabolism*
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Mitochondrial Membrane Transport Proteins / metabolism
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Mitochondrial Proton-Translocating ATPases / metabolism
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Peptide Elongation Factor 1 / metabolism
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RNA / genetics
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RNA / metabolism*
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RNA Interference / physiology
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RNA Transport / physiology*
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RNA, Mitochondrial
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RNA, Transfer / genetics
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RNA, Transfer / metabolism*
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Tetracycline / pharmacology
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Trypanosoma brucei brucei / genetics*
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Trypanosoma brucei brucei / metabolism*
Substances
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HSP70 Heat-Shock Proteins
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Mitochondrial Membrane Transport Proteins
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Peptide Elongation Factor 1
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RNA, Mitochondrial
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Isopropyl Thiogalactoside
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RNA
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RNA, Transfer
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Mitochondrial Proton-Translocating ATPases
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Tetracycline