Identification of non-diphtheriae corynebacterium by use of matrix-assisted laser desorption ionization-time of flight mass spectrometry

J Clin Microbiol. 2012 Jan;50(1):160-3. doi: 10.1128/JCM.05889-11. Epub 2011 Nov 9.

Abstract

We evaluated the Bruker Biotyper matrix-assisted laser desorption ionization-time of flight (MALDI-TOF) mass spectrometry for identification of 92 clinical isolates of Corynebacterium species in comparison to identification using rpoB or 16S rRNA gene sequencing. Eighty isolates (87%) yielded a score of ≥1.700, and all of these were correctly identified to the species level with the exception of Corynebacterium aurimucosum being misidentified as the closely related Corynebacterium minutissimum.

Publication types

  • Comparative Study
  • Evaluation Study

MeSH terms

  • Bacteriological Techniques / methods*
  • Corynebacterium / chemistry*
  • Corynebacterium / classification*
  • Corynebacterium / isolation & purification
  • Corynebacterium Infections / diagnosis*
  • Corynebacterium Infections / microbiology*
  • DNA, Bacterial / chemistry
  • DNA, Bacterial / genetics
  • DNA, Ribosomal / chemistry
  • DNA, Ribosomal / genetics
  • DNA-Directed RNA Polymerases / genetics
  • Humans
  • RNA, Ribosomal, 16S / genetics
  • Sensitivity and Specificity
  • Sequence Analysis, DNA
  • Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization / methods*
  • Time Factors

Substances

  • DNA, Bacterial
  • DNA, Ribosomal
  • RNA, Ribosomal, 16S
  • DNA-Directed RNA Polymerases
  • RNA polymerase beta subunit