Context: Magnesium fluoride (MgF(2)) nanoparticles-stabilized oil-in-water nanosized emulsion was prepared and assessed for its antiadherent and antibiofilm activities over glass coupons against pathogenic microorganisms like Escherichia coli and Staphylococcus aureus.
Objective: The major objectives of this paper are to synthesis MgF(2) nanoparticles, to prepare MgF(2) nanoparticles-stabilized nanosized emulsion, to coat glass coupons with MgF(2) nanoparticles and nanoparticles-stabilized emulsion, to challenge the emulsion or nanoparticles-coated and uncoated glass surfaces with fresh bacterial cultures over 18 h for biofilm formation, and to evaluate the extended antibiofilm activity of the coupons coated with either nanoparticles or emulsion in restricting the bacterial growth and biofilm formation.
Results and discussion: Uncoated surfaces supported a massive biofilm formation (12.6 × 10(11) and 11.6 × 10(11) CFU cm(-2) for E. coli and S. aureus respectively for the 3rd day) while emulsion-coated surfaces dramatically restricted bacterial colonization (9.3 and 8.0 CFU cm(-2) for E. coli and S. aureus respectively). These results suggested that MgF(2) nanoparticles-stabilized emulsion is effective in restraining bacterial colonization on glass surfaces.
Conclusion: Although the glass coupons are selected as a model biomaterial surface, similar or increased antibiofilm action would be expected when this 'hybrid' nanoparticulate system is coated on other types of biomaterial surfaces such as intraocular lenses, catheters, etc.