Abstract
We present the coupled use of specifically localized fluorescent gene markers and image processing for automated quantitative analysis of cell growth and genetic activity across living plant tissues. We used fluorescent protein markers to identify cells, create seeds and boundaries for the automatic segmentation of cell geometries and ratiometrically measure gene expression cell by cell in Arabidopsis thaliana.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Arabidopsis / cytology
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Arabidopsis / genetics*
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Cell Membrane / genetics
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Cell Membrane / physiology
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Computational Biology
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Cytokinins / pharmacology
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Gene Expression Regulation, Developmental
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Gene Expression Regulation, Plant
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Microscopy, Confocal
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Microscopy, Fluorescence
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Plants, Genetically Modified / cytology
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Plants, Genetically Modified / genetics*
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Seeds / cytology
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Seeds / genetics*
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Seeds / ultrastructure