A chemo-enzymatic approach for site-specific modification of the RNA cap

Angew Chem Int Ed Engl. 2013 Jul 22;52(30):7874-8. doi: 10.1002/anie.201302874. Epub 2013 Jun 21.

Abstract

Capped and gowned: A two-step approach can be used to site-specifically modify the 5'-cap of eukaryotic mRNAs. First, a trimethylguanosinesynthase variant recognizes the m(7)G cap structure and introduces bioorthogonal groups using S-adenosyl-L-methionine-based cosubstrates. Then, the enzymatically introduced reporter groups are further modified by thiol-ene or CuAAC click chemistry (see scheme).

Keywords: AdoMet analogues; click chemistry; mRNA; trimethylguanosinesynthase.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Click Chemistry
  • Humans
  • Methyltransferases / metabolism*
  • Molecular Structure
  • RNA Caps / chemistry*
  • RNA Caps / metabolism
  • RNA, Messenger / chemistry*
  • RNA, Messenger / metabolism
  • S-Adenosylmethionine / chemistry*
  • Substrate Specificity

Substances

  • RNA Caps
  • RNA, Messenger
  • S-Adenosylmethionine
  • Methyltransferases
  • trimethylguanosine synthase