Bioorthogonal site-specific labeling of the 5'-cap structure in eukaryotic mRNAs

Chem Commun (Camb). 2014 May 4;50(34):4478-81. doi: 10.1039/c4cc01549e. Epub 2014 Mar 24.

Abstract

We present a chemo-enzymatic approach for site-specific labeling of 5'-capped RNAs based on bioorthogonal chemistry. A trimethylguanosine synthase was engineered to transfer a terminal azido moiety to the 5'-cap which could be further modified using strain-promoted azide-alkyne cycloaddition (SPAAC).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Alkynes / chemistry
  • Azides / chemistry
  • Carbocyanines / chemistry
  • Cycloaddition Reaction
  • Giardia / enzymology
  • Methyltransferases / genetics
  • Methyltransferases / metabolism
  • RNA, Messenger / chemistry*
  • Rhodamines / chemistry

Substances

  • Alkynes
  • Azides
  • Carbocyanines
  • RNA, Messenger
  • Rhodamines
  • cyanine dye 5
  • lissamine rhodamine B
  • Methyltransferases
  • trimethylguanosine synthase