Abstract
We present a chemo-enzymatic approach for site-specific labeling of 5'-capped RNAs based on bioorthogonal chemistry. A trimethylguanosine synthase was engineered to transfer a terminal azido moiety to the 5'-cap which could be further modified using strain-promoted azide-alkyne cycloaddition (SPAAC).
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Alkynes / chemistry
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Azides / chemistry
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Carbocyanines / chemistry
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Cycloaddition Reaction
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Giardia / enzymology
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Methyltransferases / genetics
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Methyltransferases / metabolism
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RNA, Messenger / chemistry*
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Rhodamines / chemistry
Substances
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Alkynes
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Azides
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Carbocyanines
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RNA, Messenger
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Rhodamines
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cyanine dye 5
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lissamine rhodamine B
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Methyltransferases
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trimethylguanosine synthase