ORAC and other in vitro methods have to date proved useful in measuring antioxidant potential in foods. In order to better understand the potential relationship between diet and free radical production/mitigation, an in vivo analytic method can provide new insight into directly measuring reactive oxidant species (ROS). Dihydrorhodamine-6G (DHR6G) is indiscriminate to the various free radicals found in humans, and therefore can be useful in quantifying total ROS in vivo. Our aim was to investigate whether the total ROS in human subjects can be quantified using DHR6G after intake of a blend of antioxidants-rich fruit and vegetable-based materials. Twelve participants were given 100 mg of a proprietary blend of fruit, vegetable, and herb powders and concentrates commercially marketed under the trade name "Spectra™". Blood samples were collected at 0, 60, 120 and 180 min and were subsequently tested for ROS in serum using DHR6G as a fluorescent probe. By quantifying this fluorescence, we were able to measure ROS concentrations in human blood. This method is both reliable and efficient for evaluating the efficacy of antioxidants against ROS in vivo. Our data indicate that eleven participants responded to the intake of Spectra™ by significant decreases of ROS concentrations.
Keywords: DHR6G; fluorescence probe; free radicals; fruit and vegetable extracts; spectra.