Rapid Identification of OXA-48 and OXA-163 Subfamilies in Carbapenem-Resistant Gram-Negative Bacilli with a Novel Immunochromatographic Lateral Flow Assay

J Clin Microbiol. 2016 Nov;54(11):2832-2836. doi: 10.1128/JCM.01175-16. Epub 2016 Aug 17.

Abstract

We assessed a novel immunochromatographic lateral flow assay for direct identification of OXA-48-like carbapenemases and accurate differentiation of allele variants with distinct substrate profiles (OXA-48 or OXA-163 subfamilies). The assay allowed rapid (less than 4 min) and reliable direct confirmation of OXA-163- and/or OXA-48-like enzymes (with 100% sensitivity and 100% specificity) from cultured colonies that were recovered from both solid medium and spiked blood culture bottles.

MeSH terms

  • Chromatography, Affinity / methods*
  • Gram-Negative Bacteria / enzymology*
  • Microbial Sensitivity Tests / methods*
  • Sensitivity and Specificity
  • Time Factors
  • beta-Lactam Resistance*
  • beta-Lactamases / analysis*

Substances

  • beta-Lactamases

Grants and funding

Work at Coris BioConcept and at CHU UCL Dinant-Godinne was supported in part by a grant from the Region Wallonne under the CWALity convention n81318265, project FEAR (Fighting Enterobacteriaceae Antibiotic Resistance).