The goal of this study was to identify therapist and organizational characteristics associated with participation in evidence-based practice (EBP) training initiatives sponsored by a large publicly funded mental health system. Self-report data from therapists (N = 247) nested within 28 mental health clinics was collected in 2015. Results from regression analyses indicated that length of time employed at an organization was associated with individual therapist participation in an EBP initiative. Organizational and implementation climate were associated with organizational participation in an EBP initiative. Organizations characterized by higher levels of stress were more likely to participate in EBP initiatives and organizations characterized by higher engagement and educational support for EBPs were less likely to participate. Implications include the need for systems to consider organizational characteristics when sponsoring organizations in EBP initiatives.