Simultaneous Quantitation of Boric Acid and Calcium Fructoborate in Dietary Supplements by HPTLC-Densitometry

Anal Sci. 2017;33(6):743-746. doi: 10.2116/analsci.33.743.

Abstract

The paper describes a new, simple, selective and precise high-performance thin-layer chromatographic (HPTLC) method for the simultaneous identification and quantitative determination of boric acid (BA) and calcium fructoborate (CFB) in bulk and tablet/capsule dosage forms of dietary supplements. HPTLC silica gel G 60 F254 precoated glass plates were used as the stationary phase. The mobile phase consisted of 2-propanol-water 8:2 (v/v). The two boron-based compounds were adequately separated with the Rf values of 0.83 ± 0.01 (BA) and 0.59 ± 0.01 (CFB).

Keywords: Boric acid; HPTLC; calcium fructoborate; dietary supplements.

MeSH terms

  • Borates / analysis*
  • Boric Acids / analysis*
  • Chromatography, Thin Layer
  • Densitometry*
  • Dietary Supplements*
  • Fructose / analogs & derivatives*
  • Fructose / analysis

Substances

  • Borates
  • Boric Acids
  • calcium fructoborate
  • Fructose
  • boric acid