Abstract
The conformation of adenine nucleotides bound to bovine mitochondrial F1-ATPase was investigated using transfer nuclear Overhauser enhancement measurements. It is shown that all nucleotides investigated adopt a predominantly anti conformation when bound to the catalytic sites. Furthermore, the experiment suggests that 8-azido-ADP and 8-azido-ATP, which are predominantly in the syn conformation in solution, are in the anti conformation when bound to F1 catalytic sites.
MeSH terms
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Adenine Nucleotides / metabolism*
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Adenosine Diphosphate / analogs & derivatives
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Adenosine Diphosphate / metabolism
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Adenosine Triphosphate / analogs & derivatives
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Adenosine Triphosphate / metabolism
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Adenylyl Imidodiphosphate / metabolism
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Animals
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Azides / metabolism
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Binding Sites
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Cattle
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Magnesium / pharmacology
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Magnetic Resonance Spectroscopy
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Mitochondria, Heart / enzymology*
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Molecular Conformation
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Proton-Translocating ATPases / metabolism*
Substances
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Adenine Nucleotides
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Azides
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Adenylyl Imidodiphosphate
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8-azidoadenosine 5'-triphosphate
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Adenosine Diphosphate
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2-azidoadenosine 3',5'-diphosphate
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2-azidoadenosine 5'-triphosphate
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Adenosine Triphosphate
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Proton-Translocating ATPases
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8-azidoadenosine diphosphate
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Magnesium