One-step nucleic acid amplification CK19 copy number for sentinel node biopsy in breast cancer: Identification of new cutoffs to predict nonsentinel axillary node involvement

Giulio Cuffolo; Harvinder K Gahir-Atwal; Brendan Smith

doi:10.1111/tbj.13977

One-step nucleic acid amplification CK19 copy number for sentinel node biopsy in breast cancer: Identification of new cutoffs to predict nonsentinel axillary node involvement

Breast J. 2020 Oct;26(10):2002-2005. doi: 10.1111/tbj.13977. Epub 2020 Jul 17.

Authors

Giulio Cuffolo¹, Harvinder K Gahir-Atwal², Brendan Smith³

Affiliations

¹ Department of Breast Surgery, Oxford University Hospitals NHS Foundation Trust, Oxford, UK.
² Department of Pathology, Royal Berkshire NHS Foundation Trust, Reading, UK.
³ Department of Breast Surgery, Royal Berkshire NHS Foundation Trust, Reading, UK.

PMID: 32677735
DOI: 10.1111/tbj.13977

Abstract

We evaluated novel sentinel node values in breast cancer for one-step nucleic acid amplification (OSNA) to predict further nodal involvement using various methods in clinically node-negative disease with a positive OSNA result and subsequent axillary node dissection. 239 patients (118 macrometastatic) were assessed revealing cutoffs of total tumor load (TTL) 44 500 copies/µL (AUROC 0.793); average copy number (ACN) 9450 (AUROC 0.790); and highest copy number (HCN) 46,000. For macrometastatic patients only: TTL 221 400 copies/µL (AUROC 0.685); ACN 64,000 (AUROC 0.671); HCN 59 500 (AUROC 0.529). Our data favor TTL and represent one of the largest OSNA macrometastatic predictive series.

Keywords: CK19; breast cancer; one-step nucleic acid amplification; total tumor load.

MeSH terms

Breast Neoplasms* / genetics
Breast Neoplasms* / surgery
DNA Copy Number Variations
Female
Humans
Lymph Nodes
Lymphatic Metastasis
Nucleic Acids*
Sentinel Lymph Node Biopsy

Substances

Nucleic Acids