Differences in leaf anatomy determines temperature response of leaf hydraulic and mesophyll CO2 conductance in phylogenetically related C4 and C3 grass species

Abstract

Leaf hydraulic and mesophyll CO₂ conductance are both influenced by leaf anatomical traits, however it is poorly understood how the temperature response of these conductances differs between C₄ and C₃ species with distinct leaf anatomy. This study investigated the temperature response of leaf hydraulic conductance (K_leaf ), stomatal (g_s ) and mesophyll (g_m ) conductance to CO₂ , and leaf anatomical traits in phylogenetically related Panicum antidotale (C₄ ) and P. bisulcatum (C₃ ) grasses. The C₄ species had lower hydraulic conductance outside xylem (K_ox ) and K_leaf compared with the C₃ species. However, the C₄ species had higher g_m compared with the C₃ species. Traits associated with leaf water movement, K_leaf and K_ox , increased with temperature more in the C₃ than in the C₄ species, whereas traits related to carbon uptake, A_net and g_m , increased more with temperature in the C₄ than the C₃ species. Our findings demonstrate that, in addition to a CO₂ concentrating mechanism, outside-xylem leaf anatomy in the C₄ species P. antidotale favours lower water movement through the leaf and stomata that provides an additional advantage for greater leaf carbon uptake relative to water loss with increasing leaf temperature than in the C₃ species P. bisulcatum.

Keywords: C4 and C3 photosynthesis; leaf hydraulic conductance; leaf hydraulic conductance inside xylem; leaf hydraulic conductance outside xylem; mesophyll conductance to CO2; temperature response.