Genome Sequence of the Virulent Model Herpes Simplex Virus 1 Strain McKrae Demonstrates the Presence of at Least Two Widely Used Variant Strains

Daniel W Renner; Lance Parsons; Jacob T Shreve; Esteban A Engel; Chad V Kuny; Lynn Enquist; Donna Neumann; Colleen Mangold; Moriah L Szpara

doi:10.1128/MRA.01146-19

Genome Sequence of the Virulent Model Herpes Simplex Virus 1 Strain McKrae Demonstrates the Presence of at Least Two Widely Used Variant Strains

Microbiol Resour Announc. 2021 Mar 25;10(12):e01146-19. doi: 10.1128/MRA.01146-19.

Authors

Daniel W Renner^{1

2}, Lance Parsons³, Jacob T Shreve², Esteban A Engel⁴, Chad V Kuny^{1

2}, Lynn Enquist⁵, Donna Neumann⁶, Colleen Mangold^{1

7}, Moriah L Szpara^{8

2}

Affiliations

¹ Department of Biology, Pennsylvania State University, University Park, Pennsylvania, USA.
² Department of Biochemistry and Molecular Biology, Center for Infectious Disease Dynamics, Huck Institutes of the Life Sciences, Pennsylvania State University, University Park, Pennsylvania, USA.
³ Lewis-Sigler Institute for Integrative Genomics, Princeton University, Princeton, New Jersey, USA.
⁴ Princeton Neuroscience Institute, Princeton University, Princeton, New Jersey, USA.
⁵ Department of Molecular Biology, Princeton University, Princeton, New Jersey, USA.
⁶ Department of Ophthalmology and Visual Sciences, University of Wisconsin School of Medicine and Public Health, Madison, Wisconsin, USA.
⁷ Department of Entomology, Pennsylvania State University, University Park, Pennsylvania, USA.
⁸ Department of Biology, Pennsylvania State University, University Park, Pennsylvania, USA moriah@psu.edu.

Abstract

Herpes simplex virus 1 (HSV-1) strain McKrae was isolated in 1965 and has been utilized by many laboratories. Three HSV-1 strain McKrae stocks have been sequenced previously, revealing discrepancies in key genes. We sequenced the genome of HSV-1 strain McKrae from the laboratory of James M. Hill to better understand the genetic differences between isolates.

Grants and funding

R01 AI132692/AI/NIAID NIH HHS/United States