Mass spectrometry analysis of circulating breast cancer cells from a Xenograft mouse model

Cinzia Donato; Katarzyna Buczak; Alexander Schmidt; Nicola Aceto

doi:10.1016/j.xpro.2021.100480

Mass spectrometry analysis of circulating breast cancer cells from a Xenograft mouse model

STAR Protoc. 2021 Apr 17;2(2):100480. doi: 10.1016/j.xpro.2021.100480. eCollection 2021 Jun 18.

Authors

Cinzia Donato¹, Katarzyna Buczak², Alexander Schmidt², Nicola Aceto^{1

3}

Affiliations

¹ Department of Biomedicine, Cancer Metastasis Laboratory, University of Basel and University Hospital Basel, 4058 Basel, Switzerland.
² Proteomics Core Facility Biozentrum, University of Basel, 4056 Basel, Switzerland.
³ Department of Biology, Institute of Molecular Health Sciences, Swiss Federal Institute of Technology (ETH) Zurich, 8093 Zurich, Switzerland.

Abstract

Circulating tumor cells (CTCs) are precursors of metastasis in various cancer types. Many aspects regarding CTC biology remain poorly understood. Here, we describe mass spectrometric analysis of CTCs from a breast cancer xenograft mouse model, including procedures comprising CTC enrichment, separation of different CTC subpopulations, and their quantitative proteomic assessment. This protocol aims to facilitate the identification of protein content dynamics in human CTCs that are physiologically shed from tumor-bearing xenografts, providing a framework for investigating metastasis biology. For complete details on the use and execution of this protocol, please refer to Donato et al. (2020).

Keywords: Cancer; Cell Biology; Cell isolation; Flow Cytometry/Mass Cytometry; Mass Spectrometry; Model Organisms; Proteomics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Breast Neoplasms / chemistry*
Female
Heterografts
Humans
Mass Spectrometry / methods*
Mice
Neoplasm Transplantation
Neoplastic Cells, Circulating / chemistry*
Proteome / analysis*
Proteome / chemistry
Proteomics

Substances

Proteome