Purification and NMR characterization of acyl carrier protein

J Biol Chem. 1987 Mar 15;262(8):3685-9.

Abstract

The acyl carrier protein preparation obtained using the 2-propanol method of Rock and Cronan (Rock, C. O., and Cronan, J. E., Jr. (1981) Methods Enzymol. 71, 341-351) can be further purified with reversed-phase high-performance liquid chromatography. A homogeneous sample of acyl carrier protein is obtained as determined by NMR and reversed-phase high-performance liquid chromatography.

Publication types

  • Research Support, U.S. Gov't, P.H.S.

MeSH terms

  • Acyl Carrier Protein / isolation & purification*
  • Acyl Carrier Protein / metabolism
  • Chromatography, High Pressure Liquid
  • Escherichia coli / metabolism
  • Magnetic Resonance Spectroscopy / methods
  • Oxidation-Reduction
  • Protein Conformation

Substances

  • Acyl Carrier Protein