Introduction: Current bioinks for 3D bioprinting, such as gelatin-methacryloyl, are generally low viscosity fluids at room temperature, requiring specialized systems to create complex geometries.
Methods and results: Adding decellularized extracellular matrix microparticles derived from porcine tracheal cartilage to gelatin-methacryloyl creates a yield stress fluid capable of forming self-supporting structures. This bioink blend performs similarly at 25°C to gelatin-methacryloyl alone at 15°C in linear resolution, print fidelity, and tensile mechanics.
Conclusion: This method lowers barriers to manufacturing complex tissue geometries and removes the need for cooling systems.
Keywords: 3D bioprinting; extracellular matrix; hydrogel; larynx; tissue engineering; trachea.