Polishing De Novo Nanopore Assemblies of Bacteria and Eukaryotes With FMLRC2

Abstract

As the accuracy and throughput of nanopore sequencing improve, it is increasingly common to perform long-read first de novo genome assemblies followed by polishing with accurate short reads. We briefly introduce FMLRC2, the successor to the original FM-index Long Read Corrector (FMLRC), and illustrate its performance as a fast and accurate de novo assembly polisher for both bacterial and eukaryotic genomes.

Keywords: de novo assembly; nanopore; polishing.