Digital subtraction angiography (DSA) is a X-ray based imaging modality for interventional procedures, and remains criterion standard for diagnosing vascular diseases. The imaging protocol of DSA involves administration of a foreign contrast medium into the blood vessel that opacifies the vasculature during the imaging. Using two-dimensional (2D) DSA with high temporal resolution, it was recently demonstrated that the pulsatile velocity can be estimated by evaluating the temporal and spatial variations of the contrast medium distributions in the blood vessel. In this paper, we evaluate the feasibility to estimate the pulsatile flow velocity using the four-dimensional (4D) DSA. To overcome the noise and artefacts of 4D-DSA data, a empirical mode decomposition plus autocorrelation based method is proposed to estimate the pulsatile velocities, and the pulsatile velocities estimated using 2D-DSA are used as reference for comparison.Clinical Relevance-4D-DSA encompasses both structural and temporal information; it theoretically reduces the need of multiple scans, hence reducing the radiation doses. The estimated pulsatile flow velocities open up a new parameter for hemodynamic studies and potential for real-time diagnostic and therapeutic monitoring during interventional procedures.