Closed genome sequence of Clostridium botulinum type B1 strain isolated from an infant botulism case in the United States

Christine A Pillai; Nagarajan Thirunavukkarasu; Narjol Gonzalez-Escalona; David Melka; Phillip Curry; Rachel Binet; Sandra Tallent; Eric Brown; Shashi Sharma

doi:10.1128/mra.00854-23

Closed genome sequence of Clostridium botulinum type B1 strain isolated from an infant botulism case in the United States

Microbiol Resour Announc. 2024 Feb 15;13(2):e0085423. doi: 10.1128/mra.00854-23. Epub 2024 Jan 5.

Affiliation

¹ Division of Microbiology, Office of Regulatory Science, Center for Food Safety and Applied Nutrition, U.S. Food and Drug Administration, College Park, Maryland, USA.

^# Contributed equally.

Abstract

We present the closed genome sequence of the Clostridium botulinum BT-22100019 strain isolated from the stool specimen of an infant diagnosed with botulism. With 4.33-Mb genome size and 28.0% G + C content, the bont/B1 gene encoded for botulinum neurotoxin serotype B was found on a 262 kb plasmid arranged in a ha⁺ orfx ^- cluster.

Keywords: Clostridium botulinum; MiSeq; genomic characterization; illumina; infant botulism; minion; nanopore; short-read sequencing.