The more we search, the more we find: discovering and expanding the biodiversity in the ring nematode genus Xenocriconemella De Grisse and Loof, 1965 (Nematoda: Criconematidae)

A Archidona-Yuste; I Clavero-Camacho; A N Ruiz-Cuenca; C Cantalapiedra-Navarrete; G Liebanas; P Castillo; J E Palomares-Rius

doi:10.1186/s40851-024-00230-3

The more we search, the more we find: discovering and expanding the biodiversity in the ring nematode genus Xenocriconemella De Grisse and Loof, 1965 (Nematoda: Criconematidae)

Zoological Lett. 2024 Mar 25;10(1):8. doi: 10.1186/s40851-024-00230-3.

Authors

A Archidona-Yuste^#¹, I Clavero-Camacho^#², A N Ruiz-Cuenca^{2

3}, C Cantalapiedra-Navarrete², G Liebanas³, P Castillo², J E Palomares-Rius²

Affiliations

¹ Department of Crop Protection, Institute for Sustainable Agriculture (IAS), Spanish National Research Council (CSIC), Avda. Menendez Pidal s/n, Campus de Excelencia Internacional Agroalimentario, ceiA3, 14004, Córdoba, Spain. antonio.archidona@ias.csic.es.
² Department of Crop Protection, Institute for Sustainable Agriculture (IAS), Spanish National Research Council (CSIC), Avda. Menendez Pidal s/n, Campus de Excelencia Internacional Agroalimentario, ceiA3, 14004, Córdoba, Spain.
³ Department of Animal, Plant Biology and Ecology, University of Jaén, Campus Las Lagunillas, Jaén, Spain.

^# Contributed equally.

Abstract

The ring nematode genus Xenocriconemella De Grisse and Loof, 1965 comprises only one nominal species, Xenocriconemella macrodora (Taylor, 1936) De Grisse and Loof, 1965. The initial objective of the present study was to investigate the morphological-morphometric and molecular diversity of 28 X. macrodora populations in the Iberian Peninsula associated with tree forests (mainly Quercus spp.). However, a detailed integrative taxonomic analysis (morphological-morphometric and molecular data) from each population and analysis of this data using principal component analysis (PCA) for morphometric data (including these 28 populations and other 25 X. macrodora populations around the world) and molecular and phylogenetic species delimitation methods revealed that X. macrodora forms a species complex. This species complex is composed by species that are morphometricly and morphologically similar, but clearly different at the molecular level. Three new species are described applying integrative taxonomy, namely as Xenocriconemella iberica sp. nov., Xenocriconemella paraiberica sp. nov. and Xenocriconemella pradense sp. nov. However, the molecular diversity of this species in USA and Italy confirmed that additional species are likely present in this species complex, and the diversity of this group may be higher than expected. The study of X. macrodora topotypes can clarify the position of this species using molecular markers under an integrative approach.

Keywords: Integrative taxonomy; Morphometry; Ribosomal and mitochondrial markers; Species complex.

Abstract

Grants and funding