Methods for Generating Self-Organizing Human Patterned Heart Organoids Using Pluripotent Stem Cells

Brett Volmert; Aitor Aguirre

doi:10.1007/7651_2024_545

Methods for Generating Self-Organizing Human Patterned Heart Organoids Using Pluripotent Stem Cells

Methods Mol Biol. 2024 Apr 23. doi: 10.1007/7651_2024_545. Online ahead of print.

Authors

Brett Volmert^{1

2}, Aitor Aguirre^{3

4}

Affiliations

¹ Institute for Quantitative Health Science and Engineering, Division of Developmental and Stem Cell Biology, Michigan State University, East Lansing, MI, USA.
² Department of Biomedical Engineering, College of Engineering, Michigan State University, East Lansing, MI, USA.
³ Institute for Quantitative Health Science and Engineering, Division of Developmental and Stem Cell Biology, Michigan State University, East Lansing, MI, USA. aaguirre@msu.edu.
⁴ Department of Biomedical Engineering, College of Engineering, Michigan State University, East Lansing, MI, USA. aaguirre@msu.edu.

PMID: 38647861
DOI: 10.1007/7651_2024_545

Abstract

Organoids derived from pluripotent stem cells exhibit notable similarities to organ development in vitro. Nonetheless, cardiac organoids generated to date possess immature phenotypes and are unable to model the full spectrum of heart development and disease. Here, we describe the developmental maturation of human heart organoids by controlled exposure to metabolic and hormonal factors over a 10-day period, mirroring key stages of human cardiac development and resulting in significant molecular, cellular, morphological, and functional changes. Overall, our findings represent a significant advancement in synthetic human heart development, offering a valuable platform for studying cardiac disease states and conducting pharmacological research.

Keywords: Cardiac development; Cardiovascular; Heart; Human; Organoid; Pluripotent stem cell; Self-organization.