Investigating cryopreserved PBMC functionality in an antigen-induced model of sarcoidosis granuloma formation

Sarah G Seman; Sabahattin Bicer; Mark W Julian; Jonah R Mitchell; Patrick J Kramer; Elliott D Crouser; Landon W Locke

doi:10.1016/j.bbrc.2024.149993

Investigating cryopreserved PBMC functionality in an antigen-induced model of sarcoidosis granuloma formation

Biochem Biophys Res Commun. 2024 Jun 25:714:149993. doi: 10.1016/j.bbrc.2024.149993. Epub 2024 Apr 23.

Authors

Sarah G Seman¹, Sabahattin Bicer², Mark W Julian³, Jonah R Mitchell⁴, Patrick J Kramer⁵, Elliott D Crouser⁶, Landon W Locke⁷

Affiliations

¹ Biomedical Engineering Department, The Ohio State University College of Engineering, The Ohio State University, Columbus, OH, USA. Electronic address: seman.40@osu.edu.
² Biomedical Engineering Department, The Ohio State University College of Engineering, The Ohio State University, Columbus, OH, USA. Electronic address: bicer.1@osu.edu.
³ Division of Pulmonary, Critical Care and Sleep Medicine, The Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, OH, USA. Electronic address: mark.julian@osumc.edu.
⁴ Biomedical Engineering Department, The Ohio State University College of Engineering, The Ohio State University, Columbus, OH, USA. Electronic address: mitchell.1714@osu.edu.
⁵ Division of Pulmonary, Critical Care and Sleep Medicine, The Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, OH, USA. Electronic address: patrick.kramer@osumc.edu.
⁶ Division of Pulmonary, Critical Care and Sleep Medicine, The Dorothy M. Davis Heart and Lung Research Institute, The Ohio State University Wexner Medical Center, Columbus, OH, USA. Electronic address: elliott.crouser@osumc.edu.
⁷ Biomedical Engineering Department, The Ohio State University College of Engineering, The Ohio State University, Columbus, OH, USA. Electronic address: locke.51@osu.edu.

PMID: 38663096
DOI: 10.1016/j.bbrc.2024.149993

Abstract

Sarcoidosis, a systemic inflammatory disease, poses challenges in understanding its etiology and variable clinical courses. Despite ongoing uncertainty about causative agents and genetic predisposition, granuloma formation remains its hallmark feature. To address this, we developed a validated in vitro human granuloma model using patient-derived peripheral blood mononuclear cells (PBMCs), offering a dynamic platform for studying early granuloma formation and sarcoidosis pathogenesis. However, a current limitation of this model is its dependence on freshly isolated PBMCs obtained from whole blood. While cryopreservation is a common method for long-term sample preservation, the biological effects of freezing and thawing PBMCs on granuloma formation remain unclear. This study aimed to assess the viability and functionality of cryopreserved sarcoidosis PBMCs within the granuloma model, revealing similar granulomatous responses to fresh cells and highlighting the potential of cryopreserved PBMCs as a valuable tool for studying sarcoidosis and related diseases.

Keywords: Cryopreservation; Disease modeling; Granuloma formation; Peripheral blood mononuclear cells; Sarcoidosis.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Adult
Antigens / immunology
Cell Survival
Cells, Cultured
Cryopreservation*
Female
Granuloma* / immunology
Granuloma* / pathology
Humans
Leukocytes, Mononuclear* / immunology
Leukocytes, Mononuclear* / metabolism
Male
Sarcoidosis* / immunology
Sarcoidosis* / pathology

Substances

Antigens