Applying 3D ED/MicroED workflows toward the next frontiers

Mahira Aragon; Sarah E J Bowman; Chun Hsing Chen; M Jason de la Cruz; Daniel A Decato; Edward T Eng; Kristen M Flatt; Sahil Gulati; Yuchen Li; Charles J Lomba; Brandon Mercado; Jessalyn Miller; Lukáš Palatinus; William J Rice; David Waterman; Christina M Zimanyi

doi:10.1107/S2053229624004078

Applying 3D ED/MicroED workflows toward the next frontiers

Acta Crystallogr C Struct Chem. 2024 Jun 1. doi: 10.1107/S2053229624004078. Online ahead of print.

Authors

Mahira Aragon¹, Sarah E J Bowman², Chun Hsing Chen³, M Jason de la Cruz⁴, Daniel A Decato⁵, Edward T Eng¹, Kristen M Flatt⁶, Sahil Gulati⁷, Yuchen Li⁸, Charles J Lomba⁹, Brandon Mercado¹⁰, Jessalyn Miller¹, Lukáš Palatinus¹¹, William J Rice¹², David Waterman¹³, Christina M Zimanyi¹

Affiliations

¹ Simons Electron Microscopy Center, New York Structural Biology Center, 89 Convent Ave, New York, New York 10027, USA.
² Hauptman-Woodward Medical Research Institute, 700 Ellicott St, Buffalo, New York 14203, USA.
³ Department of Chemistry, University of North Carolina at Chapel Hill, Chapel Hill, North Carolina 27514, USA.
⁴ Memorial Sloan Kettering Cancer Center, 1275 York Avenue, New York, New York 10065, USA.
⁵ Chemistry and Biochemistry, University of Montana, 32 Campus Drive, Missoula, Montana 59812, USA.
⁶ Materials Research Laboratory, University of Illinois at Urbana Champaign, Urbana, Illinois 61801, USA.
⁷ Gatan Inc., Pleasanton, CA 94588, USA.
⁸ Department of Chemistry, Princeton University, Princeton, New Jersey 08544, USA.
⁹ Department of Physics, Quantitative Biology Institute, Yale University, 260 Whitney Ave., New Haven, Connecticut 06520-8103, USA.
¹⁰ Department of Chemistry, Yale University, New Haven, Connecticut 06520, USA.
¹¹ Institute of Physics of the CAS/NanED, Na Slovance 1999/2, Prague 192000, Czech Republic.
¹² Department of Cell Biology, NYU Grossman School of Medicine, 540 First Ave, New York, New York 10016, USA.
¹³ Research Complex at Harwell, UKRI-STFC Rutherford Appleton Laboratory, Harwell, Didcot, Oxfordshire, OX11 0FA, England, United Kingdom.

PMID: 38712546
DOI: 10.1107/S2053229624004078

Abstract

We report on the latest advancements in Microcrystal Electron Diffraction (3D ED/MicroED), as discussed during a symposium at the National Center for CryoEM Access and Training housed at the New York Structural Biology Center. This snapshot describes cutting-edge developments in various facets of the field and identifies potential avenues for continued progress. Key sections discuss instrumentation access, research applications for small molecules and biomacromolecules, data collection hardware and software, data reduction software, and finally reporting and validation. 3D ED/MicroED is still early in its wide adoption by the structural science community with ample opportunities for expansion, growth, and innovation.

Keywords: 3D ED; MicroED; electron diffraction; microcrystal electron diffraction.

open access.

Abstract

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