The functional role of Nudt2 in human triple negative breast cancer

Rasha Abu-Rahmah; Hovav Nechushtan; Sanaa Hidmi; Amichay Meirovitz; Ehud Razin; Tamar Peretz

doi:10.3389/fonc.2024.1364663

The functional role of Nudt2 in human triple negative breast cancer

Front Oncol. 2024 Apr 23:14:1364663. doi: 10.3389/fonc.2024.1364663. eCollection 2024.

Authors

Rasha Abu-Rahmah^{1

2}, Hovav Nechushtan¹, Sanaa Hidmi², Amichay Meirovitz³, Ehud Razin², Tamar Peretz¹

Affiliations

¹ Department of Oncology, Hadassah Hebrew University Medical Center, Jerusalem, Israel.
² Department of Biochemistry and Molecular Biology, Institute for Medical Research Israel-Canada, the Hebrew University of Jerusalem, Jerusalem, Israel.
³ Department of Oncology, Soroka-Ben Gurion University Medical Center, Beer-Sheeva, Israel.

Abstract

The main known function of Nudix hydrolase 2 (Nudt2) is to hydrolyze the secondary messenger diadenosine 5', 5'''-p1, p4-tetraphosphate (Ap4A). In this study we examined the role of Nudt2 in breast carcinoma through its expression in human invasive ductal carcinoma tissues, and its functions in human triple negative breast cancer (TNBC) cell lines. A significantly higher expression of Nudt2 was observed in human invasive ductal carcinoma tissues compared to that in normal breast tissue. Knockdown of Nudt2 in TNBC cell lines resulted in a significant reduction in cellular proliferation via the Ki67 marker, accompanied by G0/G1 phase cell cycle arrest, in the migration and invasion of these cells and in tumorigenicity and anchorage-independent growth. It can therefore be concluded that Nudt2 plays a significant role in promoting TNBC growth.

Keywords: Anchorage-independent growth; NUDT2; invasion; migration; triple negative breast cancer (TNBC).

Grants and funding

The author(s) declare financial support was received for the research, authorship, and/or publication of this article. This work was supported by the Israel Science Foundation, 115/20 (E.R).