Exosomes secreted by adipose mesenchymal stem cells overexpressing circPIP5K1C exert

Xin Gu; Li Liang; Chao Lu; Jiangyi Wang; Bao Hua; Wengfeng Li; Yuanshen Mao; Qing Yang; Bin Xu

doi:10.1016/j.bbadis.2024.167223

Exosomes secreted by adipose mesenchymal stem cells overexpressing circPIP5K1C exert

Biochim Biophys Acta Mol Basis Dis. 2024 May 6;1870(6):167223. doi: 10.1016/j.bbadis.2024.167223. Online ahead of print.

Authors

Xin Gu¹, Li Liang², Chao Lu¹, Jiangyi Wang³, Bao Hua¹, Wengfeng Li¹, Yuanshen Mao¹, Qing Yang⁴, Bin Xu⁵

Affiliations

¹ Department of Urology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201999, China.
² Department of Respiratory and Critical Care Medicine, Shanghai General Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 200080, China.
³ Department of Urology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201999, China. Electronic address: anytime@shsmu.edu.cn.
⁴ Department of Urology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201999, China. Electronic address: dryangq@gmail.com.
⁵ Department of Urology, Shanghai Ninth People's Hospital, Shanghai Jiao Tong University School of Medicine, Shanghai 201999, China. Electronic address: chxb2004@126.com.

PMID: 38718844
DOI: 10.1016/j.bbadis.2024.167223

Abstract

Background: Erectile dysfunction (ED) seriously affects men's normal life, and obstructive sleep apnoea (OSA) has been diagnosed as a causative factor. Currently, exosomes secreted by adipose mesenchymal stem cells (ADSC) have been used in the non-clinical experimental treatment of ED disease with prominent efficacy due to the advantages of high stability and no immune exclusion.

Methods: In this study, chronic intermittent hypoxia (CIH) exposure was used to induce ED-corresponding phenotypes in Sprague Dawley (SD) rats as well as in cavernous smooth muscle cells (CCSMCs). ED symptoms were treated using exosomes secreted by ADSCs overexpressing circPIP5K1C (EXO-circ) injected into the rat corpus cavernosum.

Results: EXO-circ has the effect of ameliorating ED induced by CIH exposure in rats, the mechanism of which is to promote the expression of the downstream target gene SMURF1 after adsorption of miR-153-3p through the sponge so that SMURF1 and PFKFB3 occur protein-protein binding and ubiquitination degradation of PFKFB3 appears to inhibit the occurrence of spongiotic smooth muscle cells glycolysis, and to restore the function of the smooth muscle.

Conclusions: These findings show that EXO-circ have a promising therapeutic potential in OSA-induced ED.

Keywords: Chronic intermittent hypoxia; Erectile dysfunction; Exosomes.