XA21-mediated resistance to Xanthomonas oryzae pv. oryzae is dose dependent

Nan Zhang; Xiaoou Dong; Rashmi Jain; Deling Ruan; Artur Teixeira de Araujo Junior; Yan Li; Anna Lipzen; Joel Martin; Kerrie Barry; Pamela C Ronald

doi:10.7717/peerj.17323

XA21-mediated resistance to Xanthomonas oryzae pv. oryzae is dose dependent

PeerJ. 2024 May 6:12:e17323. doi: 10.7717/peerj.17323. eCollection 2024.

Authors

Nan Zhang^#^{1

2}, Xiaoou Dong^#^{2

3

4

5}, Rashmi Jain², Deling Ruan^{2

5}, Artur Teixeira de Araujo Junior², Yan Li^{2

6}, Anna Lipzen⁷, Joel Martin⁷, Kerrie Barry⁷, Pamela C Ronald^{2

4

5}

Affiliations

¹ Key Laboratory of Seed Innovation, Institute of Genetics and Developmental Biology, Chinese Academy of Sciences, Beijing, China.
² Department of Plant Pathology and the Genome Center, University of California, Davis, CA, USA.
³ State Key Laboratory for Crop Genetics and Germplasm Enhancement and Utilization, Jiangsu Engineering Research Center for Plant Genome Editing, Nanjing Agricultural University, Nanjing, China.
⁴ Innovative Genomics Institute, University of California, Berkeley, CA, USA.
⁵ Feedstocks Division, The Joint Bioenergy Institute, Emeryville, CA, USA.
⁶ Rice Research Institute and Key Lab for Major Crop Diseases, Sichuan Agricultural University, Chengdu, China.
⁷ DOE Joint Genome Institute, Lawrence Berkeley National Laboratory, Berkeley, CA, USA.

^# Contributed equally.

Abstract

The rice receptor kinase XA21 confers broad-spectrum resistance to Xanthomonas oryzae pv. oryzae (Xoo), the causal agent of rice bacterial blight disease. To investigate the relationship between the expression level of XA21 and resulting resistance, we generated independent HA-XA21 transgenic rice lines accumulating the XA21 immune receptor fused with an HA epitope tag. Whole-genome sequence analysis identified the T-DNA insertion sites in sixteen independent T0 events. Through quantification of the HA-XA21 protein and assessment of the resistance to Xoo strain PXO99 in six independent transgenic lines, we observed that XA21-mediated resistance is dose dependent. In contrast, based on the four agronomic traits quantified in these experiments, yield is unlikely to be affected by the expression level of HA-XA21. These findings extend our knowledge of XA21-mediated defense and contribute to the growing number of well-defined genomic landing pads in the rice genome that can be targeted for gene insertion without compromising yield.

Keywords: Genetic engineering; Plant defense; Receptor-like kinase; Rice; XA21; Xanthomonas oryzae pv. oryzae.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Disease Resistance* / genetics
Oryza* / genetics
Oryza* / immunology
Oryza* / microbiology
Plant Diseases* / genetics
Plant Diseases* / immunology
Plant Diseases* / microbiology
Plant Proteins* / genetics
Plant Proteins* / metabolism
Plants, Genetically Modified*
Protein Serine-Threonine Kinases
Xanthomonas* / genetics

Substances

Xa21 protein, Oryza sativa
Plant Proteins
Protein Serine-Threonine Kinases

Supplementary concepts

Xanthomonas oryzae

Grants and funding

The work (proposal: 10.46936/10.25585/60001021) conducted by the US Department of Energy Joint Genome Institute, a DOE Office of Science User Facility, is supported by the Office of Science of the US Department of Energy operated under Contract No. DE-AC02-05CH11231. Nan Zhang is also supported by a scholarship offered by the Chinese Scholarship Council. Xiaoou Dong is also supported by the Agricultural Science and Technology Innovation Program of Jiangsu Province [CX(22)3153]. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.