Molecular cloning of the gene for dihydrofolate reductase from Lactobacillus casei

Gene. 1982 Feb;17(2):229-33. doi: 10.1016/0378-1119(82)90077-4.

Abstract

The Lactobacillus casei gene for dihydrofolate reductase has been cloned in Escherichia coli using the multicopy vector pBR322. A restriction map of the cloned DNA has been prepared. The cloned DNA directs the synthesis of L. casei dihydrofolate reductase in E. coli and confers trimethoprim and methotrexate resistance.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Chromosome Mapping
  • Cloning, Molecular
  • DNA Restriction Enzymes / metabolism
  • DNA, Bacterial / genetics
  • Escherichia coli / genetics
  • Genes
  • Lacticaseibacillus casei / genetics*
  • Tetrahydrofolate Dehydrogenase / genetics*

Substances

  • DNA, Bacterial
  • Tetrahydrofolate Dehydrogenase
  • DNA Restriction Enzymes