Genetic analysis suggests functional interactions between the N- and C-terminal domains of the TetA(C) efflux pump encoded by pBR322

P McNicholas; M McGlynn; G G Guay; D M Rothstein

doi:10.1128/jb.177.18.5355-5357.1995

Genetic analysis suggests functional interactions between the N- and C-terminal domains of the TetA(C) efflux pump encoded by pBR322

J Bacteriol. 1995 Sep;177(18):5355-7. doi: 10.1128/jb.177.18.5355-5357.1995.

Authors

P McNicholas¹, M McGlynn, G G Guay, D M Rothstein

Affiliation

¹ Department of Microbial Genetics and Biochemistry, Lederle Laboratories, Pearl River, New York 10965, USA.

Abstract

Genetic analysis of the tetA(C) gene of pBR322 indicates the importance of two-cytoplasmic loops in the TetA(C) protein (P. McNicholas, I. Chopra, and D. M. Rothstein, J. Bacteriol. 174:7926-7933, 1992). In this study, we characterized second-site suppressor mutations that suggest a functional interaction between these two cytoplasmic regions of the protein.

MeSH terms

Amino Acid Sequence
Antiporters / genetics
Antiporters / metabolism*
Bacterial Proteins / genetics
Bacterial Proteins / metabolism*
Codon / genetics
Cytoplasm
Escherichia coli / drug effects
Escherichia coli / genetics
Genes, Bacterial / genetics
Molecular Sequence Data
Plasmids / genetics*
Point Mutation
Suppression, Genetic
Tetracycline / pharmacology
Tetracycline Resistance / genetics

Substances

Antiporters
Bacterial Proteins
Codon
tetA protein, Bacteria
Tetracycline