A model for the membrane topology of the NS3 protein as predicted from the sequence of segment 10 of epizootic haemorrhagic disease virus serotype 1

Abstract

Segment 10, encoding nonstructural proteins 3 (NS3) and 3a (NS3a) of epizootic haemorrhagic disease virus serotype 1 (EHDV-1) was sequenced. Computer motif recognition programs were used for interpretation of the sequence data to predict a structure for NS3. Integral membrane protein theories were then applied to produce a general topological model for the EHDV-1 NS3 protein. Homology was observed between EHDV-1 NS3 integral membrane motifs and those similarly observed in the cognate proteins of other orbiviruses.