Purification of subunit B of Shiga toxin using a synthetic trisaccharide-based affinity matrix

V Pozsgay; L Trinh; J Shiloach; J B Robbins; A Donohue-Rolfe; S B Calderwood

doi:10.1021/bc9500711

Purification of subunit B of Shiga toxin using a synthetic trisaccharide-based affinity matrix

Bioconjug Chem. 1996 Jan-Feb;7(1):45-55. doi: 10.1021/bc9500711.

Authors

V Pozsgay¹, L Trinh, J Shiloach, J B Robbins, A Donohue-Rolfe, S B Calderwood

Affiliation

¹ Laboratory of Developmental and Molecular Immunity, National Institute of Child Health and Human Development, National Institutes of Health, Bethesda, Maryland 20892, USA.

PMID: 8741990
DOI: 10.1021/bc9500711

Abstract

The blood group P1 antigenic trisaccharide (3), which is the receptor-binding ligand of Shiga-like toxins, is synthesized in a spacer-equipped form (32) from 2-(trimethylsilyl)ethyl glucoside 5 and the 1-thiogalactoside building blocks 10 and 22 in a stereocontrolled, stepwise fashion. Covalent attachment of 32 to hydrazine group-containing agarose gel by reductive amination provided the P1 trisaccharide-containing affinity sorbent which was used for preparative scale isolation of subunit B of Shiga toxin.

MeSH terms

Automation
Bacterial Toxins / chemistry
Bacterial Toxins / isolation & purification*
Carbohydrate Conformation
Carbohydrate Sequence
Chromatography, Affinity / methods
Enterotoxins / chemistry
Enterotoxins / isolation & purification
Gels
Glycosides
Humans
Indicators and Reagents
Magnetic Resonance Spectroscopy
Molecular Sequence Data
P Blood-Group System
Sepharose
Shiga Toxins
Trisaccharides / chemical synthesis*
Trisaccharides / chemistry
Vibrio cholerae

Substances

Bacterial Toxins
Enterotoxins
Gels
Glycosides
Indicators and Reagents
P Blood-Group System
Shiga Toxins
Trisaccharides
Sepharose