Detection and isolation of recombinant proteins based on binding affinity of reporter: protein A

Methods Mol Biol. 1997:63:103-18. doi: 10.1385/0-89603-481-X:103.

Authors

S Ståhl¹, P A Nygren, M Uhlén

Affiliation

¹ Department of Biochemistry and Biotechnology, Royal Institute of Technology, Stockholm, Sweden.

PMID: 9113645
DOI: 10.1385/0-89603-481-X:103

No abstract available

MeSH terms

Affinity Labels
Bacterial Proteins / genetics
Chromatography, Affinity
Cloning, Molecular / methods
Genes, Reporter*
Genetic Vectors
Protein Binding
Recombinant Fusion Proteins / genetics
Recombinant Fusion Proteins / isolation & purification
Recombinant Fusion Proteins / metabolism
Staphylococcal Protein A / metabolism*

Substances

Affinity Labels
Bacterial Proteins
IgG Fc-binding protein, Streptococcus
Recombinant Fusion Proteins
Staphylococcal Protein A