The role of helix VIII in the lactose permease of Escherichia coli: I. Cys-scanning mutagenesis.
Frillingos S, Ujwal ML, Sun J, Kaback HR.
Frillingos S, et al. Among authors: kaback hr.
Protein Sci. 1997 Feb;6(2):431-7. doi: 10.1002/pro.5560060220.
Protein Sci. 1997.
PMID: 9041646
Free PMC article.
Of the 34 single-Cys mutants, 26 accumulate lactose to > 70% of the steady state observed with C-less permease, and an additional 7 mutants (Gly 262-->Cys, Gly 268-->Cys, Asn 272-->Cys, Pro 280-->Cys, Asn 284-->Cys, Gly 287-->Cys, and Gly 288-->Cys) exhibi …
Of the 34 single-Cys mutants, 26 accumulate lactose to > 70% of the steady state observed with C-less permease, and an additional 7 mutan …