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Overcoming the overlap problem in the assignment of 1H NMR spectra of larger proteins by use of three-dimensional heteronuclear 1H-15N Hartmann-Hahn-multiple quantum coherence and nuclear Overhauser-multiple quantum coherence spectroscopy: application to interleukin 1 beta.
Marion D, Driscoll PC, Kay LE, Wingfield PT, Bax A, Gronenborn AM, Clore GM. Marion D, et al. Among authors: bax a. Biochemistry. 1989 Jul 25;28(15):6150-6. doi: 10.1021/bi00441a004. Biochemistry. 1989. PMID: 2675964
Measurement of three-bond, 13C'-13C beta J couplings in human ubiquitin by a triple resonance, E. COSY-type NMR technique.
Hu JS, Bax A. Hu JS, et al. Among authors: bax a. J Biomol NMR. 1998 Feb;11(2):199-203. doi: 10.1023/a:1008241904271. J Biomol NMR. 1998. PMID: 9679294
A [CO]HN(CA)CB-E.COSY pulse scheme is described for measurement of three-bond couplings, 3JC'C beta, between carbonyl and aliphatic C beta carbons in ubiquitin, uniformly enriched with 13C and 15N. ...As predicted, the new Karplus parametrization yields 3JC'C beta values s
A [CO]HN(CA)CB-E.COSY pulse scheme is described for measurement of three-bond couplings, 3JC'C beta, between carbonyl and aliphatic C
356 results