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Overexpression, purification and crystallization of BamHI endonuclease.
Jack WE, Greenough L, Dorner LF, Xu SY, Strzelecka T, Aggarwal AK, Schildkraut I. Jack WE, et al. Among authors: schildkraut i. Nucleic Acids Res. 1991 Apr 25;19(8):1825-9. doi: 10.1093/nar/19.8.1825. Nucleic Acids Res. 1991. PMID: 2030964 Free PMC article.
Cofactor requirements of BamHI mutant endonuclease E77K and its suppressor mutants.
Xu SY, Schildkraut I. Xu SY, et al. Among authors: schildkraut i. J Bacteriol. 1991 Aug;173(16):5030-5. doi: 10.1128/jb.173.16.5030-5035.1991. J Bacteriol. 1991. PMID: 1907265 Free PMC article.
A mutant BamHI endonuclease, E77K, belongs to a class of catalytic mutants that bind DNA efficiently but cleave DNA at a rate more than 10(3)-fold lower than that of the wild-type enzyme (S. Y. Xu and I. Schildkraut, J. Biol. Chem. 266:4425-4429, 1991). The preferre …
A mutant BamHI endonuclease, E77K, belongs to a class of catalytic mutants that bind DNA efficiently but cleave DNA at a rate more than 10(3 …
Isolation of BamHI variants with reduced cleavage activities.
Xu SY, Schildkraut I. Xu SY, et al. Among authors: schildkraut i. J Biol Chem. 1991 Mar 5;266(7):4425-9. J Biol Chem. 1991. PMID: 1999426 Free article.
Greenough, L. F. Dorner, S. Y. Xu, T. Strezelecka, A. K. Aggarwal, and I. Schildkraut, submitted for publication). As expected, low-level constitutive expression of the bamhIR gene in E. coli from the Ptac promotor construct is lethal to the host unless the bamHIM g …
Greenough, L. F. Dorner, S. Y. Xu, T. Strezelecka, A. K. Aggarwal, and I. Schildkraut, submitted for publication). As expected …
Altered specificity of restriction endonuclease HinfI.
Petronzio T, Schildkraut I. Petronzio T, et al. Among authors: schildkraut i. Nucleic Acids Res. 1990 Jun 25;18(12):3666. doi: 10.1093/nar/18.12.3666. Nucleic Acids Res. 1990. PMID: 2362828 Free PMC article.
Two new restriction endonucleases from Proteus vulgaris.
Gingeras TR, Greenough L, Schildkraut I, Roberts RJ. Gingeras TR, et al. Among authors: schildkraut i. Nucleic Acids Res. 1981 Sep 25;9(18):4525-36. doi: 10.1093/nar/9.18.4525. Nucleic Acids Res. 1981. PMID: 6272209 Free PMC article.
61 results